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Removing and monitoring bacterial endotoxins in biological samples

bacterial endotoxins elimination and monitoring in biological samples

The removal of bacterial endotoxins from biological samples is a routine practice in many research laboratories. Gram-negative bacteria, where bacterial endotoxins come from, are characterised by their ability to grow in almost all conditions and types of samples where tests are performed in laboratories. Since the pyrogenicity of bacterial endotoxins...



Studies on the effect of bacterial endotoxins on asthma and other respiratory conditions

studies on the effect of bacterial endotoxins on asthma

Bacterial endotoxins are lipopolysaccharides (LPS) that may be present in high concentrations in what is known as organic dust and contaminated air. LPS are derived from the cell wall of Gram-negative bacteria and, given their ubiquity, it is very likely that the entire population is exposed to inhaling them even...



The (1, 3) -β-D-glucan and its interference in the LAL test

beta glucans and its interference in the lal test

The (1,3) -β-D-glucan is a polysaccharide found in a variety of plants, fungi and bacteria. This polymer can be found substituted in different positions of the chain, and occurs naturally in different configurations based on the species, depending on the substituents which have the structure and their spatial position relative...



The LAL Test: Precautions to take when conducting research into pharmaceuticals

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For many decades, it has been found that the most effective method for determining pyrogens in pharmaceutical samples, regardless of the form of the active substance, is the LAL (Limulus Amebocyte Lysate) test. Using the LAL test, the presence of bacterial endotoxins, lipopolysaccharide molecules (LPS) that are part of the...



Principles on which the Limulus test for the detection of bacterial endotoxins is based

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The LAL or Limulus test is used for the determination of bacterial endotoxins in a wide variety of samples in both research laboratories and industries. The principle of this test is based on the process of coagulation which occurs in the hemolymph of horseshoe crab (Limulus Polyphemus) in the presence...



The relationship between the chemical nature of bacterial endotoxins and their toxicity and immunogenicity

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The chemical nature of bacterial endotoxins has an effect on the toxicity that these endotoxins cause in other organisms, as well as on the immunogenic effects generated in the organism. In general, bacterial endotoxins are complexes formed by lipopolysaccharide molecules found in the cell membranes of Gram-negative bacteria. The diseases...



6 types of diseases in humans related to endotoxins of Gram-negative bacteria

diseases related to endotoxins of gram negative bacteria

By entering the bloodstream, Gram-negative bacteria cause immune system reactions and lead to the activation of different chains of non-cellular reactions. One of the components of the cell wall of this family of bacteria are lipopolysaccharides, which form complexes of high molecular weight known as bacterial endotoxins, due to the...



Recent research on the issue of bacterial endotoxins

the issue of bacterial endotoxins

Bacterial endotoxins are the most common pyrogens found in pharmaceutical products. These substances are found in the outer membrane of Gram-negative bacteria and when cell lysis occurs in bacteria, they are released to the environment where the bacteria are located. In mammals, bacterial endotoxins, which are lipopolysaccharide molecules, cause various...



The advantages of using the brand PYROSTAR™ in research laboratories

products of the brand pyrostar in research laboratories

The PYROSTAR™ brand of Wako specializes in all reagents, instruments and other accessories that are necessary to perform the detection of bacterial endotoxins in different environments. During research for medicines, diseases or other branches of biomedicine, it may be necessary to perform this type of analysis. This is mainly due...



The role of lipopolysaccharides of bacterial origin in liver diseases

lipopolysaccharides of bacterial origin

Scientists who study liver diseases have found a relationship between liver diseases and bacteria as well as intestinal products. In many cases, pathogenic bacteria that are found in the gastrointestinal tract of an individual are of the Gram-negative type, whose cellular wall has, among other components, lipopolysaccharides. It is considered that...