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Interfering factors in the LAL test

interfering factors in the limulus test

The Limulus Amebocyte Lysate test, known by its acronym "LAL", is used to determine the presence of bacterial endotoxins from the cellular wall of Gram-negative bacteria. The LAL test has been carried out since the discovery, more than six decades ago, of the fact that the blood of the horseshoe...



Determination of endotoxins in the plasma via the LAL method

determination of endotoxins in the plasma

As we mentioned before in the other articles of this blog, The LAL (Limulus Amebocyte Lysate) test uses the hemolymph of the horsheshoe crab Limulus Polyphemus for the determination of bacterial endotoxins. Bacterial endotoxins are the most frequent pyrogens that can contaminate pharmacological samples and samples of other types. These...



The importance of the use of glucan in the LAL tests

use of glucan in the LAL tests

The presence of bacterial endotoxins in different media, dissolutions, laboratory material, air, among others, can be determined via the LAL (Limulus Amebocyte Lysate) test. This test has become very important in the industry and research in the last few decades, turning into the most reliable test for the detection of...



LAL Reagents for Research Use

lal kit for research use

The LAL (Limulus amebocyte lysate) test is conducted in the industry for the detection of bacterial endotoxins and pyrogens in general. However, this test is not only used at the industrial level; it is also necessary to use the LAL test in research laboratories when we need to know if...



The determination of bacterial endotoxins: How to avoid the contamination of the samples during handling

The determination of bacterial endotoxins How to avoid the contamination of the samples during handling

The handling of the samples to which the Limulus amebocyte lysate (LAL) test will be applied plays a key role in the results obtained. Bacterial endotoxins are the most frequently encountered pyrogen in the samples of medicine, foods and other products. Therefore, it is very easy for the samples that...



Comparing the LAL Method with the Pyrogen Test on Rabbits

comparing the lal method with the pyrogen test on rabbits

When the goal is to check the content of endotoxins that exist in a sample, The LAL (Limulus Amebocyte Lysate) method has largely replaced the pyrogen test that used to be conducted via the use of rabbits not only in the experiments that are conducted in research, but also in...



The Importance of Using the Brand PYROSTAR™ in Research with Pharmaceuticals

Pyrostar Logo3

The brand PYROSTAR™ of the company "Wako" specialises in reagents for the detection of bacterial endotoxins. Bacterial endotoxins, originating from the cell walls of the Gram-negative bacteria, are one of the main and most powerful sources of contamination in pharmaceuticals. What are known as bacterial endotoxins are Lipopolysaccharides (LPS) that...



The Detection of Endotoxins Via the LAL Test, the Chromogenic Method

chromogenic method

The detection of endotoxins via the LAL (LimulusAmebocyte Lysate) test is a technique that is used daily in research laboratories, above all in the laboratories where biological matters are researched, and particularly in biomedicine laboratories. This technique is also very common in the pharmaceutical industry, where it is used to...



The Detection of Endotoxins Via the LAL test, the Turbidimetric Method

turbidimetric method

In the previous article of this blog, we made reference to the Gel-Clot method to qualify endotoxins using the Limulus Amebocyte Lysate (LAL) test. This article focuses more on the turbidimetric method, which is another one of the techniques used when endotoxin analysis is conducted. As mentioned before, the Limulus Amebocyte...



The Detection of Endotoxins via the LAL Test, the Gel Clot Method

detection endotoxins lal test gel clot method

The Limulus Amebocyte Lysate test is recommended in international pharmacopoeias as the method for detecting bacterial toxins both in the raw materials used for the production of medicines and for the final products. This test is also useful for the cosmetics industry and in food production as it is the method...