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The Detection of Endotoxins Via the LAL Test, the Chromogenic Method

chromogenic method

The detection of endotoxins via the LAL (LimulusAmebocyte Lysate) test is a technique that is used daily in research laboratories, above all in the laboratories where biological matters are researched, and particularly in biomedicine laboratories. This technique is also very common in the pharmaceutical industry, where it is used to...



The Detection of Endotoxins Via the LAL test, the Turbidimetric Method

turbidimetric method

In the previous article of this blog, we made reference to the Gel-Clot method to qualify endotoxins using the Limulus Amebocyte Lysate (LAL) test. This article focuses more on the turbidimetric method, which is another one of the techniques used when endotoxin analysis is conducted. As mentioned before, the Limulus Amebocyte...



The Detection of Endotoxins via the LAL Test, the Gel Clot Method

detection endotoxins lal test gel clot method

The Limulus Amebocyte Lysate test is recommended in international pharmacopoeias as the method for detecting bacterial toxins both in the raw materials used for the production of medicines and for the final products. This test is also useful for the cosmetics industry and in food production as it is the method...



The Rabbit and the Horseshoe Crab

the rabbit and the horse shoe crab

The LAL method for endotoxin detection was largely preceded by the Rabbit Pyrogen Testing by about 30 years. Much information has already been provided on the procedures and details of the LAL endotoxin testing. The Rabbit Pyrogen Testing has mostly been outdated by the LAL procedure. Discussions about the Pyrogen Testing...



The Impact of Endotoxin on the Human Body

impact of endotoxin on the human body

Definition of Endotoxin Endotoxin is a form of sugar. It is a structure composed of lipids and sugar complexes. This structure is called a lipopolysaccharide or LPS. This structure is a necessary component for gram negative bacteria to maintain cell wall integrity. It is a major component of their cell wall and...



The necessity of LAL endotoxin testing

the necessity of lal endotoxin testing

A history of tragedies is what gave birth to the need for Limulus Amebocyte Lysate (LAL) endotoxin testing in the food and medico-pharmaceutical industry today. This is a history of unfortunate morbidities and mortalities and the role of endotoxin screening was set as a barrier to prevent these events from repeating...



Applications of the LAL test in the pharmaceutical industry

applications of the lal test in the pharmaceutical industry.jpg

In bacteria, as in all living organisms, chemical reactions of nutrient transformation that allow the formation of structures which are vital for the life of these microorganisms take place. These reactions as a whole form what we know of as the metabolism of bacteria. The lipopolysaccharides (LPS) that form part of...



The myths and facts regarding bacterial endotoxins

the myths and facts regarding bacterial endotoxins

The LAL test, also known as the Limulus Amebocyte Lysate test, is a way to determine the presence of bacterial endotoxins. This method is used commonly to check that different media are free of endotoxins and is mainly used in the pharmaceutical industry, where it has been substituted for the pyrogen...



What are the various applications of the LAL method?

the various applications of the lal method

On wakopyrostar.com, we can find various products to conduct the detection of bacterial endotoxins via the application of the LAL technique (Limulus Amebocyte Lysate). The Limulus Amebocyte Lysate technique is used for multiple applications in research studies and in the industry. In this article, we will describe some of these applications. Bacterial...



The LAL Method: First Steps and at Present

The LAL Method First Steps and at Present3

In the 1950’s, the first studies to determine bacterial endotoxins using the LAL method began to appear after researchers headed by Frederick Bang in the Marine Biological Laboratory, Woods Hole, Massachusetts (USA).  Bang discovered that the blue-colored blood (or hemolymph) of the Atlantic Horseshoe crab (Limulus polyphemus), coagulated when exposed to...