Blog - Kit LAL


6 types of diseases in humans related to endotoxins of Gram-negative bacteria

diseases related to endotoxins of gram negative bacteria

By entering the bloodstream, Gram-negative bacteria cause immune system reactions and lead to the activation of different chains of non-cellular reactions. One of the components of the cell wall of this family of bacteria are lipopolysaccharides, which form complexes of high molecular weight known as bacterial endotoxins, due to the...



Recent research on the issue of bacterial endotoxins

the issue of bacterial endotoxins

Bacterial endotoxins are the most common pyrogens found in pharmaceutical products. These substances are found in the outer membrane of Gram-negative bacteria and when cell lysis occurs in bacteria, they are released to the environment where the bacteria are located. In mammals, bacterial endotoxins, which are lipopolysaccharide molecules, cause various...



The advantages of using the brand PYROSTAR™ in research laboratories

products of the brand pyrostar in research laboratories

The PYROSTAR™ brand of Wako specializes in all reagents, instruments and other accessories that are necessary to perform the detection of bacterial endotoxins in different environments. During research for medicines, diseases or other branches of biomedicine, it may be necessary to perform this type of analysis. This is mainly due...



Bacterial endotoxins as contaminants of therapeutic proteins

contaminants of therapeutic proteins

The purity of a protein to be used for therapeutic purposes is of vital importance. In terms of the evidence necessary to determine whether a protein will be viable as a medicine, it is essential that the protein has a high degree of purity and that it does not cause...



Interfering factors in the LAL test

interfering factors in the limulus test

The Limulus Amebocyte Lysate test, known by its acronym "LAL", is used to determine the presence of bacterial endotoxins from the cellular wall of Gram-negative bacteria. The LAL test has been carried out since the discovery, more than six decades ago, of the fact that the blood of the horseshoe...



Determination of endotoxins in the plasma via the LAL method

determination of endotoxins in the plasma

As we mentioned before in the other articles of this blog, The LAL (Limulus Amebocyte Lysate) test uses the hemolymph of the horsheshoe crab Limulus Polyphemus for the determination of bacterial endotoxins. Bacterial endotoxins are the most frequent pyrogens that can contaminate pharmacological samples and samples of other types. These...



LAL Reagents for Research Use

lal kit for research use

The LAL (Limulus amebocyte lysate) test is conducted in the industry for the detection of bacterial endotoxins and pyrogens in general. However, this test is not only used at the industrial level; it is also necessary to use the LAL test in research laboratories when we need to know if...



The determination of bacterial endotoxins: How to avoid the contamination of the samples during handling

The determination of bacterial endotoxins How to avoid the contamination of the samples during handling

The handling of the samples to which the Limulus amebocyte lysate (LAL) test will be applied plays a key role in the results obtained. Bacterial endotoxins are the most frequently encountered pyrogen in the samples of medicine, foods and other products. Therefore, it is very easy for the samples that...



Comparing the LAL Method with the Pyrogen Test on Rabbits

comparing the lal method with the pyrogen test on rabbits

When the goal is to check the content of endotoxins that exist in a sample, The LAL (Limulus Amebocyte Lysate) method has largely replaced the pyrogen test that used to be conducted via the use of rabbits not only in the experiments that are conducted in research, but also in...



The Detection of Endotoxins Via the LAL Test, the Chromogenic Method

chromogenic method

The detection of endotoxins via the LAL (LimulusAmebocyte Lysate) test is a technique that is used daily in research laboratories, above all in the laboratories where biological matters are researched, and particularly in biomedicine laboratories. This technique is also very common in the pharmaceutical industry, where it is used to...