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Kits for LAL test by using the Colorimetric Method

11th January 2019

Kits for LAL by using the Colorimetric Method The LAL test (Limulus amebocyte lysate) is a method of great importance for determination of endotoxins of bacterial origin.

In Fujifilm Wako, we have kits to perform LAL test using the colorimetric method, which include all the reagents necessary for color development that enables bacterial endotoxins detection, obtaining accurate values in a short time of analysis. You can learn more about the principles for colorimetric detection in LAL test  on our blog.

The Limulus Color KY Test of the PYROSTAR brand is a kit to perform LAL test that allows the quantitative determination of bacterial endotoxin present in a sample. This kit contains a mixture of all lyophilized reagents. In this mixture, p-nitroaniline dye is bound through a peptide bond, which causes it to be in a colorless form. When the cascade of reactions caused by endotoxins occur, p-nitroaniline is released into the medium and it produces a yellow color. Color measurement can be used as an analytical signal and it allows detecting endotoxin specifically with high sensitivity.

In Limulus Color KY series, you can find a multiple tests kit and an individual measurement kit, each of which is designed to use the kinetic colorimetric method. The use of these kits benefits the user by avoiding false positive results, and endotoxin detection can be achieved from 0.0002 EU/mL (in case of using the kit for individual measurement) and from 0.0005 EU/mL when the kit is used for multiple measurements.

The Toxinometer® ET-6000 is a device designed by Wako for the PYROSTAR product line. This tube reader allows measurements of samples that are incubated for LAL test. It is simple to use and expansion modules are available in case that the user requires to measure a number of samples greater than the 16 that are measured in a module. Analysis can be carried out at temperatures of 30°C and 37°C. Among the many advantages of acquiring this equipment is that printed copies of tests results can be obtained and that it is capable of carrying out complete analyzes without technicians intervention.

The accessories belonging to the BioClean Series of PYROSTAR brand are also very important, consisting of endotoxin-free pipette tips (<0.005EU/tip) packed in individual envelopes and with non-stick protective film, so that no dust is deposited. These tips are compatible with the Finnpipette Digital Micro Pipettor (sizes 20-200 μl or 100-1000 μl) and they can be used with the Toxinometer® ET-6000. In addition, from this same series, endotoxin-free, sterilized at 250 °C, caps and test tubes can be purchased.

The PYROSTAR brand also offers the Endotoxin Extraction Solution for LAL Tests, which avoids the use of water or saline in tests, as they are not as effective for endotoxin extraction. The endotoxin-free extracting solution is used at a 1:20 dilution and contains HSA (human serum albumin), which allows surface endotoxins to be extracted that would not be extracted by water or saline.


Toxinometer® ET-7000 Series PYROSTAR™ ES-F Single Test Vial Control Standard Endotoxin
Toxinometer® ET-7000 Series PYROSTAR™ ES-F Single Test Vial Control Standard Endotoxin

By: Lisa Komski In: Kit LAL Tags: LAL test