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Blog - Kit LAL


What Are Pyrogens?

Feb 18, 2020
what are pyrogens

Most of us have experienced a fever at some point in our lives. When our body temperature rises above its typical 98.6 F (37 C), we experience symptoms such as sweating, chills, and weakness. But why do we experience fevers, and how are they triggered by pyrogens? BIOLOGY OF THE FEVER...


By: Lisa Komski In: Kit LAL
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How Does the LAL Assay Work?

Jan 31, 2020
how does the lal assay work

Gram-negative bacteria can cause a variety of illnesses, including many types of food poisoning, pneumonia, and sexually-transmitted diseases. These bacteria contain molecules called endotoxins in their cell walls. Even in the absence of living bacteria, residual endotoxins can be deadly when they contaminate pharmaceuticals or medical devices. Contact with endotoxins...


By: Lisa Komski In: Kit LAL
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The role of lipopolysaccharides in obesity

Nov 29, 2019
the role of lipopolysaccharides in obesity

Endotoxins are lipopolysaccharides (LPS) found in the outer cell wall of Gram-negative bacteria. Bacterial endotoxins are pyrogenic, meaning that when in contact with the human body, they can induce a fever response. In high enough doses, endotoxins can lead to septic shock, which is a potentially life-threatening condition. Humans seem to...


By: Lisa Komski In: Kit LAL
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FUJIFILM WAKO Endotoxin Detection Products Now Available from Lonza

Nov 1, 2019
Copy of CHPI

FUJIFILM Wako Pure Chemical Corporation (Japan) together with its US subsidiary FUJIFILM Wako Chemicals U.S.A. Corporation announced a distribution agreement for Wako’s portfolio of endotoxin products, accessories and instrumentation in the regions of South America and Asia. Lonza will sell, support and distribute Wako’s endotoxin products in the regions of South...


By: Default Admin In: Kit LAL, Wako News
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How to avoid nanoparticle interference in the LAL Assay

Oct 22, 2019
interference nanoparticles lal test

Recent technological advances have allowed nanomaterials to be utilized for biology and medicine. Nanoparticles have unique physiochemical properties that can offer novel strategies to treat diseases. However, these nanomaterials have the potential to trigger dangerous immune reactions, which may be due to the presence of bacterial endotoxin. Endotoxin is a large,...


By: Default Admin In: Kit LAL
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The Role of Lipopolysaccharides in Autoimmune Diseases

Sep 20, 2019
the role of lps in autoimmune diseases

Lipopolysaccharide (LPS), also called endotoxin, is a phospholipid that makes up the outer cell wall of Gram-negative bacteria. A component of LPS, lipid A, can induce a fever response or even septic shock in humans. For this reason, LPS levels in pharmaceutical products and medical devices must be strictly monitored...


By: Default Admin In: Kit LAL
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Choosing the Best Detection Method for Your LAL Assay

Aug 8, 2019
best detection method for your lal test

The Limulus Amebocyte Lysate (LAL) assay is the most widely-used method for quantifying levels of bacterial endotoxins. Endotoxins are lipopolysaccharides found in the cell walls of Gram-negative bacteria. Since endotoxins can trigger dangerous inflammatory reactions upon contact with the human body, the LAL test is a vital part of quality...


By: Lisa Komski In: Kit LAL
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Where Are LAL Assays Used?

Jul 9, 2019
cases where the lal tests are used

The LAL assay (short for Limulus Amoebocyte Lysate) is the most popular method for quantifying bacterial endotoxins. Endotoxins are lipopolysaccharides that can trigger a harmful immune reaction if they enter the human body. For this reason, endotoxins must be carefully monitored to ensure that their levels are below safe thresholds....


By: Lisa Komski In: Kit LAL
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Extraction and Quantification of Bacterial Endotoxin

May 3, 2019
extraction and quantification of bacterial endotoxin

Endotoxins are lipopolysaccharide molecules found in the outer membrane of Gram-negative bacteria. Endotoxins that enter the human body can illicit a dangerous inflammatory reaction. For this reason, FDA guidelines impose strict limits on endotoxin levels in medical device extracts. Devices that contact the cardiovascular or lymphatic system must be below...


By: Lisa Komski In: Kit LAL
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Correct Ways to Use LAL accessories

Apr 17, 2019
correct ways to use lal accessories

The Limulus Amebocyte Lysate (LAL) assay is an efficient method for the detection of bacterial endotoxin in Gram-negative bacteria. It was developed in 1960 based on the clotting reaction of haemolymph from the Atlantic horseshoe crab. There are a number of LAL accessories available for bacterial endotoxin testing, for instance,...


By: Lisa Komski In: Kit LAL
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