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The use of the Turbidimetric Method in the LAL test

May 25, 2018
turbidimetric method in the lal test

The turbidimetric method is one of the methods used for the detection of the end point when endotoxin analysis is performed using the Limulus Amebocyte Lysate (LAL) test. By applying the turbidimetric method, we can conduct the LAL test in a semi-quantitative or quantitative manner.The LAL test measures the turbidity...


By: Lisa Komski In: Kit LAL
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Analytical Methods for the detection of bacterial endotoxins

Mar 15, 2018
methods for the detection of bacterial endotoxins

There are three analytical methods for the determination of bacterial endotoxins through the Limulus Amebocyte Lysate test, also known as the LAL test by its acronym. These methods are the Gel-Clot method, the turbidimetric method and the colorimetric method, which can be carried out in a qualitative or quantitative manner.Gel-Clot...


By: Lisa Komski In: Kit LAL
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The importance of having the right accessories to carry out the LAL test

Feb 16, 2018
accessories suitable for carrying out the lal test

The LAL test (Limulus Amebocyte Lysate) allows us to determine lipopolysachharides of bacterial origin. The lipopolysachharide molecules found in the outer cell wall of Gram-negative bacteria, also known as bacterial endotoxins, are released when bacterial lysis takes place. These substances have a high level of toxicity and are very stable,...


By: Lisa Komski In: Kit LAL
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Where and why is the LAL test performed?

Jan 23, 2018
where and why is the lal test performed

The LAL test (acronym for Limulus Amebocyte Lysate) is a test for the determination of bacterial endotoxins, which uses an Amebocyte Lysate of the Limulus crab. In this test, the reactions which occur in the Amebocyte Lysate as a result of a defence mechanism of the crab in the presence...


By: Lisa Komski
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Reagents and Accessories for the Limulus Amebocyte Lysate Test (LAL)

Jan 8, 2018
reagents and accessories for the limulus amebocyte lysate test

The Limulus Amebocyte Lysate Test (LAL) is a method for the detection and quantification of bacterial endotoxins that is widely used by the pharmaceutical and biotechnology industry. The biochemical principle of the test is based on the clotting reaction of the hemolymph of the Limulus polyphemus, commonly known as the...


By: Lisa Komski In: Kit LAL
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Potential applications of the Horseshoe Crab in the development of health products

Nov 9, 2017
the horseshoe crab and its importance for health

The Horseshoe Crab from the Atlantic, commonly known as Limulus polyphemus, has existed for more than 200 million years. It has been classified under the category Arthropods since the 19th century, and not under Crustaceans as it was first thought. This close relative of Arachnids has historically been harvested for...


By: Lisa Komski In: Kit LAL
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The Role of LPS in the Progression of Diabetic Nephropathy

Sep 26, 2017
lps in the progression of diabetic nephropathy

Lipopolysaccharides (LPS) or bacterial endotoxins are associated with a large range of pathologies. Some of these are tumor necrosis, neuroinflammation, bacteriobilia, respiratory diseases, coronary artery diseases, neonatal necrotizing enterocolitis, neurodegenerative diseases, cystic fibrosis, autoimmune diseases and even male infertility, as we mentioned before.In this article, we will talk about another...


By: Lisa Komski In: Kit LAL
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Male Infertility Caused by Bacterial Lipopolysaccharides

Sep 1, 2017
male infertility caused by lps

The spermatozoon is the male gamete whose function is to lead the DNA towards the egg (female gamete) and form a zygote that will later turn into the embryo and the fetus. Therefore, the sperm count is considered to be of great importance for public health primarily because it is...


By: Lisa Komski
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Association of Bacterial Lipopolysaccharides in the Pathology of Alzheimer’s Disease

Jun 30, 2017
bacterial lipopolysaccharides in alzheimers disease

Gram-negative bacteria are reported as the predominant microorganisms in the normal human brain. In previous articles, we have discussed the mechanisms that lipopolysaccharide endotoxins (LPS) possess to cause neuroinflammation and its results. However, research has gone a little further, associating these molecules with the clinical appearance of neurodegenerative diseases like...


By: Lisa Komski In: Kit LAL
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Immunomodulation by Bacterial Lipopolysaccharides

May 31, 2017
immunomodulation by bacterial lipopolysaccharides

Lipopolysaccharides (LPS) or bacterial endotoxins are a molecule that is amply found in the outer membrane of gram-negative bacteria, except for species of the Sphingomonas genus, which have glucosphingolipids instead. Although at high concentrations LPS can induce fever, increased heart rate, septic shock and death, at relatively low concentrations some...


By: Lisa Komski In: Kit LAL
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