×

This website uses cookies. By using the site you are agreeing to our Privacy Policy.

Blog


FUJIFILM WAKO Endotoxin Detection Products Now Available from Lonza

Nov 1, 2019
Copy of CHPI

FUJIFILM Wako Pure Chemical Corporation (Japan) together with its US subsidiary FUJIFILM Wako Chemicals U.S.A. Corporation announced a distribution agreement for Wako’s portfolio of endotoxin products, accessories and instrumentation in the regions of South America and Asia. Lonza will sell, support and distribute Wako’s endotoxin products in the regions of South...


By: Default Admin In: Kit LAL, Wako News
read more

How to avoid nanoparticle interference in the LAL Assay

Oct 22, 2019
interference nanoparticles lal test

Recent technological advances have allowed nanomaterials to be utilized for biology and medicine. Nanoparticles have unique physiochemical properties that can offer novel strategies to treat diseases. However, these nanomaterials have the potential to trigger dangerous immune reactions, which may be due to the presence of bacterial endotoxin. Endotoxin is a large,...


By: Default Admin In: Kit LAL
read more

The Role of Lipopolysaccharides in Autoimmune Diseases

Sep 20, 2019
the role of lps in autoimmune diseases

Lipopolysaccharide (LPS), also called endotoxin, is a phospholipid that makes up the outer cell wall of Gram-negative bacteria. A component of LPS, lipid A, can induce a fever response or even septic shock in humans. For this reason, LPS levels in pharmaceutical products and medical devices must be strictly monitored...


By: Default Admin In: Kit LAL
read more

Choosing the Best Detection Method for Your LAL Assay

Aug 8, 2019
best detection method for your lal test

The Limulus Amebocyte Lysate (LAL) assay is the most widely-used method for quantifying levels of bacterial endotoxins. Endotoxins are lipopolysaccharides found in the cell walls of Gram-negative bacteria. Since endotoxins can trigger dangerous inflammatory reactions upon contact with the human body, the LAL test is a vital part of quality...


By: Lisa Komski In: Kit LAL
read more

Where Are LAL Assays Used?

Jul 9, 2019
cases where the lal tests are used

The LAL assay (short for Limulus Amoebocyte Lysate) is the most popular method for quantifying bacterial endotoxins. Endotoxins are lipopolysaccharides that can trigger a harmful immune reaction if they enter the human body. For this reason, endotoxins must be carefully monitored to ensure that their levels are below safe thresholds....


By: Lisa Komski In: Kit LAL
read more

How to Avoid Common Mistakes with the LAL Assay

Jun 21, 2019
how to avoid common mistakes with lal assay

The Limulus amebocyte lysate (LAL) assay is the most popular method for detection and quantification of bacterial endotoxins. Care must be taken to avoid common mistakes with this assay that may lead to incorrect results. THIS ARTICLE WILL OUTLINE FOUR TIPS TO AVOID THESE COMMON PITFALLS AND ENSURE ACCURACY IN...


By: Lisa Komski
read more

Advantages of the LAL Assay

May 24, 2019
advantages of the lal

The outer membranes of Gram-negative bacteria contain liposaccharide molecules called endotoxins. Human exposure to endotoxins triggers a strong inflammatory reaction, which can be life-threatening. To avoid this, the FDA requires  that all pharmaceuticals and medical devices be tested for endotoxin levels. Medical device extracts must contain below 0.5 EU/mL if...


By: Lisa Komski
read more

Extraction and Quantification of Bacterial Endotoxin

May 3, 2019
extraction and quantification of bacterial endotoxin

Endotoxins are lipopolysaccharide molecules found in the outer membrane of Gram-negative bacteria. Endotoxins that enter the human body can illicit a dangerous inflammatory reaction. For this reason, FDA guidelines impose strict limits on endotoxin levels in medical device extracts. Devices that contact the cardiovascular or lymphatic system must be below...


By: Lisa Komski In: Kit LAL
read more

Correct Ways to Use LAL accessories

Apr 17, 2019
correct ways to use lal accessories

The Limulus Amebocyte Lysate (LAL) assay is an efficient method for the detection of bacterial endotoxin in Gram-negative bacteria. It was developed in 1960 based on the clotting reaction of haemolymph from the Atlantic horseshoe crab. There are a number of LAL accessories available for bacterial endotoxin testing, for instance,...


By: Lisa Komski In: Kit LAL
read more

Kinetic-turbidimetric LAL method for Endotoxin Detection (PYROSTAR™)

Feb 28, 2019
pyrostar kinetic turbidimetric lal method

Bacterial endotoxin is the lipopolysaccharide (LPS) component of the cell membrane of Gram-negative bacteria. The presence of endotoxin can be detected by a specific and efficient method known as the Limulus Amebocyte Lysate (LAL) assay. The LAL assay was developed in the 1960s and is based on the clotting reaction...


By: Lisa Komski In: Kit LAL
read more