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Extraction and Quantification of Bacterial Endotoxin

May 3, 2019
extraction and quantification of bacterial endotoxin

Endotoxins are lipopolysaccharide molecules found in the outer membrane of Gram-negative bacteria. Endotoxins that enter the human body can illicit a dangerous inflammatory reaction. For this reason, FDA guidelines impose strict limits on endotoxin levels in medical device extracts. Devices that contact the cardiovascular or lymphatic system must be below...

By: Lisa Komski In: Kit LAL
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Correct Ways to Use LAL accessories

Apr 17, 2019
correct ways to use lal accessories

The Limulus Amebocyte Lysate (LAL) assay is an efficient method for the detection of bacterial endotoxin in Gram-negative bacteria. It was developed in 1960 based on the clotting reaction of haemolymph from the Atlantic horseshoe crab. There are a number of LAL accessories available for bacterial endotoxin testing, for instance,...

By: Lisa Komski In: Kit LAL
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Kinetic-turbidimetric LAL method for Endotoxin Detection (PYROSTAR™)

Feb 28, 2019
pyrostar kinetic turbidimetric lal method

Bacterial endotoxin is the lipopolysaccharide (LPS) component of the cell membrane of Gram-negative bacteria. The presence of endotoxin can be detected by a specific and efficient method known as the Limulus Amebocyte Lysate (LAL) assay. The LAL assay was developed in the 1960s and is based on the clotting reaction...

By: Lisa Komski In: Kit LAL
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Kits for LAL Testing Using the Kinetic-Chromogenic Method

Jan 11, 2019
kits for lal by using the colorimetric method

The LAL Test (Limulus amebocyte lysate) is a method of great importance for the determination of endotoxins of bacterial origin. At Fujifilm Wako, we manufacture kits to perform the kinetic-chromogenic LAL assay, which include all of the reagents necessary for bacterial endotoxins detection through a change in color development, and allow...

By: Lisa Komski In: Kit LAL
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Use of Colorimetric Method in LAL test

Nov 30, 2018
colorimetric method in lal test

The colorimetric analysis methods allow, by measuring the absorbance variation of a sample at a certain wavelength, to calculate the concentration of analytes of interest. This detection method can be used in LAL test (Limulus amebocyte lysate) for endotoxins. The LAL test is a well-known technique to researchers for determination of...

By: Lisa Komski In: Kit LAL
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Kits for the LAL test via the Gel-Clot Method

Aug 30, 2018
lal kits used in the gel clot method

The amebocyte lysate extracted from the horseshoe crab is capable of detecting very little quantities of bacterial endotoxins, which makes it possible to develop kits with a very low limit of detection. The analytical signal used in the LAL test with the Gel-Clot method is gel formation. The qualitative analysis with...

By: Default Admin
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The use of the Gel-Clot Method in the LAL test

Aug 24, 2018
the use of the gel clot method in the lal test

The LAL test (Limulus Amebocyte Lysate) is used to determine the presence of bacterial endotoxins in biological samples or samples of other types. This analysis can be performed using the Gel-Clot method, or gelation for detection as a gel is formed if the test is positive. The bacteria could lead to...

By: Lisa Komski
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Kits for the LAL test with the Turbidimetric Method

May 21, 2018
kits lal test turbidimetric method

The brand PYROSTAR has different kits to conduct the LAL test, that is, for the detection of bacterial endotoxins. Some of these kits are designed to be used with the turbidimetric methods of analysis. The analytical sign that can be measured and quantified through these kits is the turbidity formed...

By: Lisa Komski In: Kit LAL
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The use of the Turbidimetric Method in the LAL test

Apr 20, 2018
turbidimetric method in the lal test

The turbidimetric method is one of the methods used for the detection of the end point when endotoxin analysis is performed using the Limulus Amebocyte Lysate (LAL) test. By applying the turbidimetric method, we can conduct the LAL test in a semi-quantitative or quantitative manner. The LAL test measures the turbidity...

By: Lisa Komski In: Kit LAL
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Analytical Methods for the detection of bacterial endotoxins

Mar 15, 2018
methods for the detection of bacterial endotoxins

There are three analytical methods for the determination of bacterial endotoxins through the Limulus Amebocyte Lysate test, also known as the LAL test by its acronym. These methods are the Gel-Clot method, the turbidimetric method and the colorimetric method, which can be carried out in a qualitative or quantitative manner. GEL-CLOT...

By: Lisa Komski In: Kit LAL
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