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Where Are LAL Assays Used?

Jul 9, 2019
cases where the lal tests are used

The LAL assay (short for Limulus Amoebocyte Lysate) is the most popular method for quantifying bacterial endotoxins. Endotoxins are lipopolysaccharides that can trigger a harmful immune reaction if they enter the human body. For this reason, endotoxins must be carefully monitored to ensure that their levels are below safe thresholds....


By: Lisa Komski In: Kit LAL
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How to Avoid Common Mistakes with the LAL Assay

Jun 21, 2019
how to avoid common mistakes with lal assay

The Limulus amebocyte lysate (LAL) assay is the most popular method for detection and quantification of bacterial endotoxins. Care must be taken to avoid common mistakes with this assay that may lead to incorrect results. THIS ARTICLE WILL OUTLINE FOUR TIPS TO AVOID THESE COMMON PITFALLS AND ENSURE ACCURACY IN...


By: Lisa Komski
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Advantages of the LAL Assay

May 24, 2019
advantages of the lal

The outer membranes of Gram-negative bacteria contain liposaccharide molecules called endotoxins. Human exposure to endotoxins triggers a strong inflammatory reaction, which can be life-threatening. To avoid this, the FDA requires  that all pharmaceuticals and medical devices be tested for endotoxin levels. Medical device extracts must contain below 0.5 EU/mL if...


By: Lisa Komski
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Extraction and Quantification of Bacterial Endotoxin

May 3, 2019
extraction and quantification of bacterial endotoxin

Endotoxins are lipopolysaccharide molecules found in the outer membrane of Gram-negative bacteria. Endotoxins that enter the human body can illicit a dangerous inflammatory reaction. For this reason, FDA guidelines impose strict limits on endotoxin levels in medical device extracts. Devices that contact the cardiovascular or lymphatic system must be below...


By: Lisa Komski In: Kit LAL
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Correct Ways to Use LAL accessories

Apr 17, 2019
correct ways to use lal accessories

The Limulus Amebocyte Lysate (LAL) assay is an efficient method for the detection of bacterial endotoxin in Gram-negative bacteria. It was developed in 1960 based on the clotting reaction of haemolymph from the Atlantic horseshoe crab. There are a number of LAL accessories available for bacterial endotoxin testing, for instance,...


By: Lisa Komski In: Kit LAL
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Kinetic-turbidimetric LAL method for Endotoxin Detection (PYROSTAR™)

Feb 28, 2019
pyrostar kinetic turbidimetric lal method

Bacterial endotoxin is the lipopolysaccharide (LPS) component of the cell membrane of Gram-negative bacteria. The presence of endotoxin can be detected by a specific and efficient method known as the Limulus Amebocyte Lysate (LAL) assay. The LAL assay was developed in the 1960s and is based on the clotting reaction...


By: Lisa Komski In: Kit LAL
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Kits for LAL Testing Using the Kinetic-Chromogenic Method

Jan 11, 2019
kits for lal by using the colorimetric method

The LAL Test (Limulus amebocyte lysate) is a method of great importance for the determination of endotoxins of bacterial origin. At Fujifilm Wako, we manufacture kits to perform the kinetic-chromogenic LAL assay, which include all of the reagents necessary for bacterial endotoxins detection through a change in color development, and allow...


By: Lisa Komski In: Kit LAL
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Use of Colorimetric Method in LAL test

Nov 30, 2018
colorimetric method in lal test

The colorimetric analysis methods allow, by measuring the absorbance variation of a sample at a certain wavelength, to calculate the concentration of analytes of interest. This detection method can be used in LAL test (Limulus amebocyte lysate) for endotoxins. The LAL test is a well-known technique to researchers for determination of...


By: Lisa Komski In: Kit LAL
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Kits for the LAL test via the Gel-Clot Method

Aug 30, 2018
lal kits used in the gel clot method

The amebocyte lysate extracted from the horseshoe crab is capable of detecting very little quantities of bacterial endotoxins, which makes it possible to develop kits with a very low limit of detection. The analytical signal used in the LAL test with the Gel-Clot method is gel formation. The qualitative analysis with...


By: Default Admin
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The use of the Gel-Clot Method in the LAL test

Aug 24, 2018
the use of the gel clot method in the lal test

The LAL test (Limulus Amebocyte Lysate) is used to determine the presence of bacterial endotoxins in biological samples or samples of other types. This analysis can be performed using the Gel-Clot method, or gelation for detection as a gel is formed if the test is positive. The bacteria could lead to...


By: Lisa Komski
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