Blog


The importance of the use of glucan in the LAL tests

Jun 8, 2015
use of glucan in the LAL tests

The presence of bacterial endotoxins in different media, dissolutions, laboratory material, air, among others, can be determined via the LAL (Limulus Amebocyte Lysate) test. This test has become very important in the industry and research in the last few decades, turning into the most reliable test for the detection of...


By: Lisa Komski
read more

LAL Reagents for Research Use

Apr 29, 2015
lal kit for research use

The LAL (Limulus amebocyte lysate) test is conducted in the industry for the detection of bacterial endotoxins and pyrogens in general. However, this test is not only used at the industrial level; it is also necessary to use the LAL test in research laboratories when we need to know if...


By: Lisa Komski In: Kit LAL
read more

The determination of bacterial endotoxins: How to avoid the contamination of the samples during handling

Mar 24, 2015
The determination of bacterial endotoxins How to avoid the contamination of the samples during handling

The handling of the samples to which the Limulus amebocyte lysate (LAL) test will be applied plays a key role in the results obtained. Bacterial endotoxins are the most frequently encountered pyrogen in the samples of medicine, foods and other products. Therefore, it is very easy for the samples that...


By: Lisa Komski In: Kit LAL
read more

Comparing the LAL Method with the Pyrogen Test on Rabbits

Feb 20, 2015
comparing the lal method with the pyrogen test on rabbits

When the goal is to check the content of endotoxins that exist in a sample, The LAL (Limulus Amebocyte Lysate) method has largely replaced the pyrogen test that used to be conducted via the use of rabbits not only in the experiments that are conducted in research, but also in...


By: Lisa Komski In: Kit LAL
read more

The Importance of Using the Brand PYROSTAR™ in Research with Pharmaceuticals

Jan 29, 2015
Pyrostar Logo3

The brand PYROSTAR™ of the company "Wako" specialises in reagents for the detection of bacterial endotoxins. Bacterial endotoxins, originating from the cell walls of the Gram-negative bacteria, are one of the main and most powerful sources of contamination in pharmaceuticals. What are known as bacterial endotoxins are Lipopolysaccharides (LPS) that...


By: Lisa Komski In: Wako News
read more

The Detection of Endotoxins Via the LAL Test, the Chromogenic Method

Dec 16, 2014
chromogenic method

The detection of endotoxins via the LAL (LimulusAmebocyte Lysate) test is a technique that is used daily in research laboratories, above all in the laboratories where biological matters are researched, and particularly in biomedicine laboratories. This technique is also very common in the pharmaceutical industry, where it is used to...


By: Lisa Komski In: Kit LAL
read more

The Detection of Endotoxins Via the LAL test, the Turbidimetric Method

Nov 27, 2014
turbidimetric method

In the previous article of this blog, we made reference to the Gel-Clot method to qualify endotoxins using the Limulus Amebocyte Lysate (LAL) test. This article focuses more on the turbidimetric method, which is another one of the techniques used when endotoxin analysis is conducted. As mentioned before, the Limulus Amebocyte...


By: Lisa Komski In: Kit LAL
read more

The Detection of Endotoxins via the LAL Test, the Gel Clot Method

Oct 8, 2014
detection endotoxins lal test gel clot method

The Limulus Amebocyte Lysate test is recommended in international pharmacopoeias as the method for detecting bacterial toxins both in the raw materials used for the production of medicines and for the final products. This test is also useful for the cosmetics industry and in food production as it is the method...


By: Lisa Komski In: Kit LAL
read more

The Rabbit and the Horseshoe Crab

Sep 23, 2014
the rabbit and the horse shoe crab

The LAL method for endotoxin detection was largely preceded by the Rabbit Pyrogen Testing by about 30 years. Much information has already been provided on the procedures and details of the LAL endotoxin testing. The Rabbit Pyrogen Testing has mostly been outdated by the LAL procedure. Discussions about the Pyrogen Testing...


By: Lisa Komski In: Kit LAL
read more

The Impact of Endotoxin on the Human Body

Aug 20, 2014
impact of endotoxin on the human body

Definition of Endotoxin Endotoxin is a form of sugar. It is a structure composed of lipids and sugar complexes. This structure is called a lipopolysaccharide or LPS. This structure is a necessary component for gram negative bacteria to maintain cell wall integrity. It is a major component of their cell wall and...


By: Lisa Komski In: Kit LAL
read more