LAL Reagent Water

LAL Reagent Water can be used to fulfill all aspects of endotoxin testing. From reconstitution of both the CSE and LAL for the creation of standard curves to the making of product dilutions, LAL reagent water provides reliable and reproducible data when paired with our endotoxin-specific LAL reagents.

Key Features

  • Endotoxin-free water (<0.001 EU/mL)
  • Great for reconstitution of LAL, RSE and CSE
  • Used to perform dilution series of RSE and CSE
  • Used for sample dilutions
Catalog Number Product Name Quantity
LRW-12100 LAL Reagent Water, 100 mL 12 x 100 mL glass bottles
LRW-2030 LAL Reagent Water, 30 mL 20 x 30 mL glass bottles
LRW-12125 LAL Reagent Water, 30 mL 12 x 125 mL plastic bottles

FAQ: LAL Reagent Water (LRW)

Q: Should the LRW react with LAL?

A: No, the LRW should not react with the LAL.

Q: How much LRW is required to dissolve the PYROSTAR™ ES-F Series and the Limulus Color KY series?

A: The amount of LRW depends on the testing format of the product.

  • PYROSTAR™ single test: 0.2 mL of LRW for the negative control only. The sample or dilutions (0.2 mL) that are being examined should be added to the single test lyophilized vial to reconstitute the reagent.
  • PYROSTAR™ multi test: 2.0 mL of LRW or 5.2 mL of LRW should be added to the vial, depending on the volume listed on the label.
  • PYROSTAR™ ES-F/Plate: Only 2.0 mL of LRW should be added according to the volume listed on the label.
  • Limulus Color KY single test: 0.2 mL of LRW for the negative control only. The sample or dilutions (0.2 mL) that are being examined should be added to the single test lyophilized vial to reconstitute the reagent.
  • Limulus Color KY multi test: Only 2.0 mL of LRW should be added according to the volume listed on the label.

Q: How much LRW is required to reconstitute CSE for a kit?

A: The easiest way to determine the volume of LRW needed to reconstitute the CSE is to check the specific LAL lot’s corresponding Certificate of Analysis (COA). For PYROSTAR™ ES-F Series and Limulus Color KY series, a COA is provided or can be accessed at www.wakopyrostar.com.

When testing with Limulus Color KY series, the potency is shown on the CSE vial label. Divide the potency (EU/vial) on the label by 1,000 to determine the volume (mL) of LRW necessary for reconstituting the CSE to yield 1,000 EU/mL.